Genetic quality assurance and genetic monitoring of laboratory murines
In the past years the number of mouse and rat models has increased dramatically. This is partly due to a variety of techniques developed to manipulate the genome in a targeted manner, but also to the subsequent breeding experiments to generate two-, three-, and four-fold mutants. In addition many crossing experiments have been carried out to generate a huge number of conditional mutant strains. Unfortunately, the main focus in the majority of the crossing experiments lies on the manipulated loci; whereas the genetic background of the animals is often neglected. The working group will develop recommendations for the genetic quality assurance of laboratory murines. These recommendations should be a guide to animal facilities and researchers on how animal experiments can be carried out under genetically standardized conditions so that these recommendations will also contribute to the three R’s.
Genetics, quality, mice, genotyping