Methods of Humane killing of laboratory fish

The European Directive lists some methods of humane killing for laboratory fish. Some member states, research establishments and scientific projects allow or require some other techniques, based on scientific needs, health and safety recommendations or animal welfare experimental data. For example, some fish species display averseness towards the most commonly used chemical anaesthetics. The alternatives are physical methods like electro- stunning or hypothermal shock which perceived speeds of action seem to prevent any prolonged discomfort. However, this relies on the concept that humane killing should be a fast and painless experience. The choice of techniques would depend on the species, the stage of development,staff and environment safety, the faith of the carcass, the protocol’s reliability andefficacy or animal welfare cost. The aim of the working group will be to propose good practice guidance on all available chemical and physical methods of humane killing of laboratory fish.